生长分化因子11对小鼠颞下颌关节骨关节炎髁突软骨细胞脂肪化的影响

doi:10.7518/hxkq.2022.01.003

华西口腔医学杂志 ›› 2022, Vol. 40 ›› Issue (1): 14-21.doi: 10.7518/hxkq.2022.01.003

生长分化因子11对小鼠颞下颌关节骨关节炎髁突软骨细胞脂肪化的影响

王若欣¹^,²(), 刘倩², 何峰², 张勉², 王贺林³()

^1.郑州大学口腔医学院，郑州 450052
^2.军事口腔医学国家重点实验室，国家口腔疾病临床医学研究中心，陕西省口腔疾病国际联合研究中心，第四军医大学口腔医院口腔解剖生理学教研室，西安 710032
^3.军事口腔医学国家重点实验室，国家口腔疾病临床医学研究中心，第四军医大学口腔医院医疗康复科，西安 710032

收稿日期:2020-12-02 修回日期:2021-10-25 出版日期:2022-02-01 发布日期:2022-02-07
通讯作者: 王贺林 E-mail:2927253441@qq.com;921441502@qq.com
作者简介:王若欣，学士，E-mail：2927253441@qq.com
基金资助:
国家自然科学基金(81700995);大学生创新创业训练项目(202110459049)

Effect of growth differentiation factor 11 on the steatosis of condylar chondrocytes in temporomandibular joint osteoarthritic mice

Wang Ruoxin¹^,²(), Liu Qian², He Feng², Zhang Mian², Wang Helin³()

^1.School of Stomatology, Zhengzhou University, Zhengzhou 450052, China
^2.State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi International Joint Research Center for Oral Diseases, Dept. of Oral Anatomy and Physiology, The Stomatological Hospital of the Fourth Military Medical University, Xi’an 710032, China
^3.State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Dept. of Medical Rehabi-litation, The Stomatological Hospital of the Fourth Military Medical University, Xi’an 710032, China

Received:2020-12-02 Revised:2021-10-25 Online:2022-02-01 Published:2022-02-07
Contact: Wang Helin E-mail:2927253441@qq.com;921441502@qq.com
Supported by:
The National Natural Science Foundation of China(81700995);Innovation and Entrepreneurship Trai-ning Program for College Students(202110459049);Correspondence: Wang Helin, E-mail: 921441502@qq.com

摘要/Abstract

摘要： 目的

观察生长分化因子11（GDF11）对小鼠颞下颌关节（TMJ）骨关节炎（OA）髁突软骨细胞脂肪化的影响。

方法

对雌性6周龄C57小鼠施加单侧前牙反𬌗（UAC）刺激并局部注射GDF11。取TMJ髁突软骨进行番红O染色；GDF11、脂联素（Adiponectin）免疫组织化学染色；实时定量聚合酶链式反应（real-time PCR）检测GDF11、Adiponectin、Ⅱ型胶原（Col-Ⅱ）、Aggrecan、Ⅰ型胶原（Col-Ⅰ）、脂肪酸结合蛋4（FABP4）、脂滴包被蛋白1（Perilipin 1）的mRNA含量。

结果

UAC实验组髁突软骨显著变薄、番红O阳性面积显著减少；Col-Ⅱ和Aggrecan的mRNA表达量显著降低；Adiponectin阳性细胞率显著增加；Col-Ⅰ、Adiponectin、FABP4、Perilipin 1的mRNA表达量显著上升。GDF11注射后髁突软骨显著增厚、番红O阳性面积显著增加；Col-Ⅱ和Aggrecan的mRNA表达量显著升高；Adiponectin阳性细胞率显著减少；Col-Ⅰ、Adiponectin、FABP4、Perilipin 1的mRNA表达量显著降低。

结论

UAC刺激诱导的髁突退变软骨中GDF11表达降低并存在软骨细胞脂肪化，补充外源性GDF11可有效抑制软骨细胞脂肪化。

关键词: 颞下颌关节, 骨关节炎, 生长分化因子11, 软骨细胞, 脂肪化

Abstract: Objective

To observe the effect of growth differentiation factor 11 (GDF11) on the steatosis of condylar chondrocytes in temporomandibular joint (TMJ) osteoarthritic mice.

Methods

Six-week-old female C57 mice were stimulated with unilateral anterior crossbite (UAC) and locally injected with GDF11. TMJ sections were stained with safranin O, and GDF11 and Adiponectin in the TMJ cartilage were detected by immunohistochemical staining. GDF11, Adiponectin, type Ⅱ collagen (Col-Ⅱ), Aggrecan, type Ⅰ collagen (Col-Ⅰ), fatty acid-binding protein 4 (FABP4), and Perilipin 1 mRNA were detected by real-time polymerase chain reaction (real-time PCR).

Results

The thickness and safranin O-positive area of TMJ cartilage in the UAC groups, as well as the mRNA expression of Col-Ⅱ and aggrecan, significantly decreased. The ratio of Adiponectin-positive cells significantly increased. The mRNA expression levels of Col-Ⅰ, Adiponectin, FABP4, and Perilipin 1 significantly increased. The TMJ cartilage of the GDF11 injection groups significantly thickened, and the safranin O-positive area significantly increased. The mRNA expression of Col-Ⅱ and Aggrecan significantly increased. The ratio of Adiponectin-positive cells significantly decreased. The mRNA expression levels of Col-I, Adiponectin, FABP4, and Perilipin 1 significantly decreased.

Conclusion

Extensive steatosis of chondrocytes and decreased expression of GDF11 in the degenerated cartilage of TMJ osteoarthritis are induced by UAC stimulation. Supplementation of exogenous GDF11 can effectively inhibit the steatosis of chondrocytes.

Key words: temporomandibular joint, osteoarthritis, growth differentiation factor 11, chondrocytes, steatosis

中图分类号:

R 782.6

王若欣, 刘倩, 何峰, 张勉, 王贺林. 生长分化因子11对小鼠颞下颌关节骨关节炎髁突软骨细胞脂肪化的影响[J]. 华西口腔医学杂志, 2022, 40(1): 14-21.

Wang Ruoxin, Liu Qian, He Feng, Zhang Mian, Wang Helin. Effect of growth differentiation factor 11 on the steatosis of condylar chondrocytes in temporomandibular joint osteoarthritic mice[J]. West China Journal of Stomatology, 2022, 40(1): 14-21.

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分子	引物序列
GDF11	F：CTGCGCCTAGAGAGCATCAAG
	R：TCTCGGTGGTAGCGTGGTA
Adiponectin	F：CCCCGGAACCCCTGGCAGGAAAG
	R：GGGTCTCCAGCCCCACACTGAACG
Col-Ⅱ	F：CATCCAGGGCTCCAATGATGTA
	R：ATGTCCATGGGTGCGATGTC
Aggrecan	F：TTCCACCAGTGCGATGCAG
	R：TGGTGTCCCGGATTCCGTA
Col-Ⅰ	F：GCGAGTGCTGTGCTTTCT
	R：AGCAAAGTTTCCTCCAAGG
FABP4	F：CGCAGACGACAGGAAGGTGA
	R：TCCACCACCAGCTTGTCACC
Perilipin 1	F：TTACAGCCCTGCCCAACCCGAGAG
	R：TGCCCAGGTCACTGCGGAGATGG
GAPDH	F：TGTGTCCGTCGTGGATCTGA
	R：TTGCTGTTGAAGTCGCAGGAG

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生长分化因子11对小鼠颞下颌关节骨关节炎髁突软骨细胞脂肪化的影响

Effect of growth differentiation factor 11 on the steatosis of condylar chondrocytes in temporomandibular joint osteoarthritic mice

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