微振动对成骨细胞成骨效应的瞬时影响

doi:10.7518/hxkq.2017.01.010

摘要/Abstract

摘要：

目的在不考虑间歇期的介入所带来的延时效应下,研究高频率低振幅微振动（LMHFV）刺激对成骨细胞成骨效应的瞬时影响。方法培养小鼠成骨前体细胞系（MC3T3-E1）,实验组在施加高频率（40 Hz）、低振幅（0.49 g）微振动30 min后,立即通过实时荧光定量聚合酶链反应检测早期成骨分化标志物Runt相关转录因子2（Runx2）、Ⅰ型胶原蛋白（Col-Ⅰ）、碱性磷酸酶（ALP）的表达,并检测活性氧（ROS）浓度变化及线粒体形态变化,后加入抗氧化剂后继续观察上述指标的变化。结果 LMHFV刺激后Runx2、Col-Ⅰ、ALP的mRNA表达明显下调（P<0.01）,线粒体ROS浓度升高（P<0.01）,线粒体长度缩短（P<0.01）;抗氧化剂的使用则使上述变化得到显著缓解（P<0.01）。结论排除间歇期的干扰后,LMHFV（40 Hz,0.49 g）可降低成骨细胞内早期成骨分化标志物的表达,促进线粒体ROS的产生与积累并导致线粒体的过度分裂。

关键词: 微振动, 成骨分化, 活性氧, 线粒体形态

Abstract:

Objective This study investigated the rapid response of osteoblasts, which were derived from low-magnitude high-frequency vibration (LMHFV). Refractory period-derived memory response was also observed. Methods MC3T3-E1 cells were incubated and received LMHFV stimulation (0.49 g, 40 Hz) for 30 min. After application of LMHFV, mRNA levels of earlier osteogenic differentiation markers Runt-related transcription factor 2 (Runx2), collagen typeⅠ(Col-Ⅰ), and alkaline phosphatase (ALP) were immediately detected by real-time fluorescence quantitative polymerase chain reaction in the absence or presence of antioxidant. Simultaneously, concentrations of mitochondrial reactive oxygen species (ROS) and average mito-chondrial length were also measured. Results Osteoblasts in the vibration group showed decreased gene expressions of Runx2, Col-Ⅰ, and ALP (P<0.01) and increased levels of mitochondrial ROS (P<0.01) and shortened mitochondria (P<0.01), whereas antioxidant treatment resulted in recovery from changes in the above indicators (P<0.01). Conclusion LMHFV can downregulate mRNA levels of early osteogenic differentiation markers, promote ROS generation, and mitochondrial fission.

Key words: vibration, osteogenic differentiation, reactive oxygen species, mitochondrial morphology

中图分类号:

朱卓立, 张玲, 马瑞阳, 甘雪琦. 微振动对成骨细胞成骨效应的瞬时影响[J]. 华西口腔医学杂志, 2017, 35(1): 68-72.

Zhuoli Zhu, Ling Zhang, Ruiyang Ma, Xueqi. Gan. Influences of vibration on rapid osteogenic response of osteoblasts[J]. West China Journal of Stomatology, 2017, 35(1): 68-72.

图/表 4

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